BAIT
LNX1
LNX, MPDZ, PDZRN2, UNQ574/PRO1136
ligand of numb-protein X 1, E3 ubiquitin protein ligase
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
SUV39H2
KMT1B, RP11-2K17.2
suppressor of variegation 3-9 homolog 2 (Drosophila)
GO Process (8)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
- cellular response to hypoxia [IDA]
- chromatin assembly or disassembly [IMP]
- chromatin remodeling [IDA]
- histone H3-K9 dimethylation [ISS]
- histone H3-K9 trimethylation [ISS]
- negative regulation of circadian rhythm [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [ISS]
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Y2H-seq approach defines the human protein methyltransferase interactome.
To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and ... [more]
Nat. Methods Apr. 01, 2013; 10(4);339-42 [Pubmed: 23455924]
Throughput
- High Throughput
Additional Notes
- Hit identified by mass spec
Curated By
- BioGRID