BAIT
TSC1
LAM, TSC
tuberous sclerosis 1
GO Process (17)
GO Function (4)
GO Component (9)
Gene Ontology Biological Process
- activation of Rho GTPase activity [IDA]
- cell cycle arrest [TAS]
- cell-matrix adhesion [IMP]
- insulin receptor signaling pathway [TAS]
- negative regulation of TOR signaling [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of insulin receptor signaling pathway [IBA]
- negative regulation of translation [IMP]
- positive regulation of focal adhesion assembly [IDA]
- protein stabilization [IDA]
- rRNA export from nucleus [IMP]
- regulation of cell cycle [IBA]
- regulation of cell-matrix adhesion [IMP]
- regulation of phosphoprotein phosphatase activity [IMP]
- regulation of stress fiber assembly [IDA]
- regulation of translation [IDA]
- response to insulin [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRMT6
HRMT1L6
protein arginine methyltransferase 6
GO Process (6)
GO Function (8)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function- histone binding [IDA]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H2A-R3 specific) [IDA]
- histone methyltransferase activity (H3-R2 specific) [IDA]
- histone methyltransferase activity (H4-R3 specific) [IDA]
- protein binding [IPI]
- protein-arginine omega-N asymmetric methyltransferase activity [IDA]
- protein-arginine omega-N monomethyltransferase activity [IDA]
- histone binding [IDA]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H2A-R3 specific) [IDA]
- histone methyltransferase activity (H3-R2 specific) [IDA]
- histone methyltransferase activity (H4-R3 specific) [IDA]
- protein binding [IPI]
- protein-arginine omega-N asymmetric methyltransferase activity [IDA]
- protein-arginine omega-N monomethyltransferase activity [IDA]
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Y2H-seq approach defines the human protein methyltransferase interactome.
To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and ... [more]
Nat. Methods Apr. 01, 2013; 10(4);339-42 [Pubmed: 23455924]
Throughput
- High Throughput
Additional Notes
- Hit identified by mass spec
Curated By
- BioGRID