BAIT
NUP98
ADIR2, NUP196, NUP96
nucleoporin 98kDa
GO Process (15)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- DNA replication [IMP]
- carbohydrate metabolic process [TAS]
- cytokine-mediated signaling pathway [TAS]
- glucose transport [TAS]
- hexose transport [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- nuclear pore complex assembly [IMP]
- nuclear pore organization [NAS]
- nucleocytoplasmic transport [TAS]
- protein import into nucleus, docking [NAS]
- regulation of glucose transport [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ARFGEF2
BIG2, PVNH2, dJ1164I10.1
ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
GO Process (8)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- Golgi to plasma membrane transport [IMP]
- endomembrane system organization [IMP]
- endosome organization [IMP]
- intracellular signal transduction [IDA]
- positive regulation of GTPase activity [IBA, IDA]
- positive regulation of tumor necrosis factor production [IMP]
- receptor recycling [IDA]
- vesicle-mediated transport [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
A high-throughput approach for measuring temporal changes in the interactome.
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]
Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]
Throughput
- High Throughput
Ontology Terms
- hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Curated By
- BioGRID