BAIT

UBA3

NAE2, UBE1C, hUBA3
ubiquitin-like modifier activating enzyme 3
UPS Project
GO Process (3)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

CAPN2

CANP2, CANPL2, CANPml, mCANP
calpain 2, (m/II) large subunit
Alzheimer's Disease Project
Homo sapiens

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A high-throughput approach for measuring temporal changes in the interactome.

Kristensen AR, Gsponer J, Foster LJ

Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]

Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Curated By

  • BioGRID