ACTG1
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- adherens junction organization [TAS]
- axon guidance [TAS]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- cellular component movement [TAS]
- innate immune response [TAS]
- membrane organization [TAS]
- platelet aggregation [IMP]
- retina homeostasis [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CDKN2A
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA]
- Ras protein signal transduction [IEP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- apoptotic mitochondrial changes [IMP]
- cell cycle arrest [IDA, IMP]
- cellular senescence [IMP]
- mitotic cell cycle [TAS]
- negative regulation of B cell proliferation [ISS]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of cell proliferation [IDA, IMP]
- negative regulation of cell-matrix adhesion [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of immature T cell proliferation in thymus [ISS]
- negative regulation of phosphorylation [IDA]
- negative regulation of protein kinase activity [IMP]
- negative regulation of transcription, DNA-templated [IMP]
- negative regulation of ubiquitin-protein transferase activity [ISS]
- positive regulation of DNA damage response, signal transduction by p53 class mediator [IDA]
- positive regulation of cell cycle arrest [IDA]
- positive regulation of cellular senescence [IMP]
- positive regulation of macrophage apoptotic process [ISS]
- positive regulation of protein sumoylation [IMP]
- positive regulation of smooth muscle cell apoptotic process [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- protein K63-linked ubiquitination [IDA]
- protein destabilization [IDA]
- protein polyubiquitination [IDA]
- protein stabilization [IDA]
- regulation of G2/M transition of mitotic cell cycle [IMP]
- regulation of apoptotic DNA fragmentation [IMP]
- regulation of protein export from nucleus [IMP]
- regulation of protein stability [ISS]
- replicative senescence [IMP]
- senescence-associated heterochromatin focus assembly [IMP]
- somatic stem cell division [ISS]
Gene Ontology Molecular Function- MDM2/MDM4 family protein binding [IPI]
- NF-kappaB binding [IDA]
- cyclin-dependent protein serine/threonine kinase inhibitor activity [IDA]
- p53 binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- transcription factor binding [IPI]
- ubiquitin-protein transferase inhibitor activity [ISS]
- MDM2/MDM4 family protein binding [IPI]
- NF-kappaB binding [IDA]
- cyclin-dependent protein serine/threonine kinase inhibitor activity [IDA]
- p53 binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- transcription factor binding [IPI]
- ubiquitin-protein transferase inhibitor activity [ISS]
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
A high-throughput approach for measuring temporal changes in the interactome.
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]
Throughput
- High Throughput
Ontology Terms
- hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CDKN2A ACTG1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID