BAIT
GCLM
GLCLR, RP4-561L24.2
glutamate-cysteine ligase, modifier subunit
GO Process (11)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- cellular nitrogen compound metabolic process [TAS]
- glutamate metabolic process [IDA]
- glutathione biosynthetic process [IDA, IMP, TAS]
- glutathione derivative biosynthetic process [TAS]
- positive regulation of glutamate-cysteine ligase activity [IBA]
- regulation of blood vessel size [IMP]
- response to drug [IDA]
- response to oxidative stress [IDA]
- small molecule metabolic process [TAS]
- sulfur amino acid metabolic process [TAS]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GNAI2
GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
GO Process (11)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- adenosine receptor signaling pathway [IBA]
- adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway [IBA, TAS]
- blood coagulation [TAS]
- cell division [IMP]
- gamma-aminobutyric acid signaling pathway [IBA]
- negative regulation of adenylate cyclase activity [TAS]
- platelet activation [TAS]
- response to nutrient [TAS]
- signal transduction [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
A high-throughput approach for measuring temporal changes in the interactome.
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]
Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]
Throughput
- High Throughput
Ontology Terms
- hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Curated By
- BioGRID