BAIT
VCPIP1
DUBA3, VCIP135
valosin containing protein (p97)/p47 complex interacting protein 1
GO Process (3)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
LARP7
ALAZS, PIP7S, HDCMA18P
La ribonucleoprotein domain family, member 7
GO Process (0)
GO Function (2)
GO Component (4)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
A high-throughput approach for measuring temporal changes in the interactome.
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]
Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]
Throughput
- High Throughput
Ontology Terms
- cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Curated By
- BioGRID