BAIT

SH3GLB2

PP6569, PP9455, RRIG1, RP11-167N5.3
SH3-domain GRB2-like endophilin B2
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

LZTFL1

BBS17
leucine zipper transcription factor-like 1
Homo sapiens

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A high-throughput approach for measuring temporal changes in the interactome.

Kristensen AR, Gsponer J, Foster LJ

Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]

Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Curated By

  • BioGRID