Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A high-throughput approach for measuring temporal changes in the interactome.

Kristensen AR, Gsponer J, Foster LJ

Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]

Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BYSL RPS27
Co-crystal Structure
Co-crystal Structure

Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Low-BioGRID
3644869

Curated By

  • BioGRID