GNB2L1
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of Wnt signaling pathway [ISS]
- negative regulation of cell growth [IDA]
- negative regulation of gene expression [IMP]
- negative regulation of hydrogen peroxide-induced neuron death [IGI]
- negative regulation of phagocytosis [IMP]
- negative regulation of protein kinase B signaling [IMP]
- negative regulation of protein tyrosine kinase activity [IDA]
- negative regulation of translation [ISS]
- positive regulation of GTPase activity [IDA]
- positive regulation of apoptotic process [IDA, IMP]
- positive regulation of cAMP catabolic process [IMP]
- positive regulation of cell migration [IDA]
- positive regulation of cyclic-nucleotide phosphodiesterase activity [IMP]
- positive regulation of gastrulation [ISS]
- positive regulation of intrinsic apoptotic signaling pathway [IMP]
- positive regulation of mitochondrial depolarization [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein homooligomerization [IDA, IMP]
- positive regulation of protein phosphorylation [IDA]
- regulation of cell cycle [IDA]
- regulation of cell division [ISS]
- regulation of establishment of cell polarity [ISS]
- regulation of protein localization [ISS]
Gene Ontology Molecular Function- SH2 domain binding [IDA]
- cysteine-type endopeptidase activator activity involved in apoptotic process [IMP]
- enzyme binding [IPI]
- ion channel inhibitor activity [ISS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein complex scaffold [TAS]
- protein homodimerization activity [IDA]
- protein kinase C binding [IDA]
- protein phosphatase binding [IPI]
- protein tyrosine kinase inhibitor activity [IDA]
- receptor binding [NAS]
- receptor tyrosine kinase binding [IDA]
- SH2 domain binding [IDA]
- cysteine-type endopeptidase activator activity involved in apoptotic process [IMP]
- enzyme binding [IPI]
- ion channel inhibitor activity [ISS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein complex scaffold [TAS]
- protein homodimerization activity [IDA]
- protein kinase C binding [IDA]
- protein phosphatase binding [IPI]
- protein tyrosine kinase inhibitor activity [IDA]
- receptor binding [NAS]
- receptor tyrosine kinase binding [IDA]
Gene Ontology Cellular Component
RPS3
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IBA, IDA]
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cellular protein metabolic process [TAS]
- cellular response to DNA damage stimulus [IEP]
- cytoplasmic translation [IBA]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- negative regulation of DNA repair [IMP]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- positive regulation of DNA N-glycosylase activity [IDA]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of apoptotic signaling pathway [IDA]
- translation [IC, NAS, TAS]
- translational elongation [TAS]
- translational initiation [NAS, TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function- DNA-(apurinic or apyrimidinic site) lyase activity [IDA]
- NF-kappaB binding [IPI]
- damaged DNA binding [IDA]
- enzyme binding [IPI]
- iron-sulfur cluster binding [NAS]
- mRNA binding [IDA]
- oxidized purine nucleobase lesion DNA N-glycosylase activity [IBA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase A binding [IPI]
- protein kinase binding [IPI]
- structural constituent of ribosome [IDA, NAS]
- DNA-(apurinic or apyrimidinic site) lyase activity [IDA]
- NF-kappaB binding [IPI]
- damaged DNA binding [IDA]
- enzyme binding [IPI]
- iron-sulfur cluster binding [NAS]
- mRNA binding [IDA]
- oxidized purine nucleobase lesion DNA N-glycosylase activity [IBA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase A binding [IPI]
- protein kinase binding [IPI]
- structural constituent of ribosome [IDA, NAS]
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
A high-throughput approach for measuring temporal changes in the interactome.
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]
Throughput
- High Throughput
Ontology Terms
- hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GNB2L1 RPS3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3365878 | |
| GNB2L1 RPS3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.4597 | BioGRID | 3219864 | |
| RPS3 GNB2L1 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | - | |
| RPS3 GNB2L1 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3756289 |
Curated By
- BioGRID