BAIT

WHI3

mRNA-binding protein WHI3, L000002486, YNL197C
RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci; regulates genes involved in the cell cycle, sister chromatid cohesion, and stress response; acts as a cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; Tpk1p (PKA) mediated phosphorylation (S568) inhibits Whi3p function, decreasing its interaction with CLN3 mRNA; regulates ploidy
Saccharomyces cerevisiae (S288c)
PREY

PRM7

YDL038C, pheromone-regulated protein PRM7, YDL039C
Pheromone-regulated protein; predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Whi3, an S. cerevisiae RNA-Binding Protein, Is a Component of Stress Granules That Regulates Levels of Its Target mRNAs.

Holmes KJ, Klass DM, Guiney EL, Cyert MS

RNA binding proteins (RBPs) are vital to the regulation of mRNA transcripts, and can alter mRNA localization, degradation, translation, and storage. Whi3 was originally identified in a screen for small cell size mutants, and has since been characterized as an RBP. The identification of Whi3-interacting mRNAs involved in mediating cellular responses to stress suggested that Whi3 might be involved in ... [more]

PLoS ONE Jan. 05, 2014; 8(12);e84060 [Pubmed: 24386330]

Throughput

  • Low Throughput

Additional Notes

  • mRNAs were high confidence interactors with Whi3p if their false discovery rate was less than 1% in two datasets and they contained a Whi3-interacting motif

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
WHI3 PRM7
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Low-BioGRID
-

Curated By

  • BioGRID