BAIT

DCP2

PSU1, decapping enzyme complex catalytic subunit DCP1, L000002866, YNL118C
Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; also enters the nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

NOG1

YPL093W
Putative GTPase; associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The polysome-associated proteins Scp160 and Bfr1 prevent P body formation under normal growth conditions.

Weidner J, Wang C, Prescianotto-Baschong C, Estrada AF, Spang A

Numerous mRNAs are degraded in processing bodies (P bodies) in S. cerevisiae. In logarithmically growing cells only 0-1 P bodies per cell are detectable. However, the number and appearance of P bodies change once the cell encounters stress. The polysome-associated mRNA binding protein Scp160 interacts with P body components such as the decapping protein Dcp2 and the scaffold protein Pat1, ... [more]

J. Cell. Sci. Feb. 25, 2014; 0(0); [Pubmed: 24569876]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NOG1 DCP2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1333BioGRID
1955075

Curated By

  • BioGRID