BAIT

SCD6

LSM13, L000001806, YPR129W
Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

EAP1

YKL204W
eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The polysome-associated proteins Scp160 and Bfr1 prevent P body formation under normal growth conditions.

Weidner J, Wang C, Prescianotto-Baschong C, Estrada AF, Spang A

Numerous mRNAs are degraded in processing bodies (P bodies) in S. cerevisiae. In logarithmically growing cells only 0-1 P bodies per cell are detectable. However, the number and appearance of P bodies change once the cell encounters stress. The polysome-associated mRNA binding protein Scp160 interacts with P body components such as the decapping protein Dcp2 and the scaffold protein Pat1, ... [more]

J. Cell. Sci. Feb. 25, 2014; 0(0); [Pubmed: 24569876]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EAP1 SCD6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2037BioGRID
2144887

Curated By

  • BioGRID