BAIT

SCD6

LSM13, L000001806, YPR129W
Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

YRA1

SHE11, RNA-binding protein YRA1, L000002959, L000002873, YDR381W
Nuclear polyadenylated RNA-binding protein; required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; interacts with DBP2; inhibits the helicase activity of Dbp2; functionally redundant with Yra2p, another REF family member
GO Process (3)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The polysome-associated proteins Scp160 and Bfr1 prevent P body formation under normal growth conditions.

Weidner J, Wang C, Prescianotto-Baschong C, Estrada AF, Spang A

Numerous mRNAs are degraded in processing bodies (P bodies) in S. cerevisiae. In logarithmically growing cells only 0-1 P bodies per cell are detectable. However, the number and appearance of P bodies change once the cell encounters stress. The polysome-associated mRNA binding protein Scp160 interacts with P body components such as the decapping protein Dcp2 and the scaffold protein Pat1, ... [more]

J. Cell. Sci. Feb. 25, 2014; 0(0); [Pubmed: 24569876]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YRA1 SCD6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1276BioGRID
1972462

Curated By

  • BioGRID