STK25
Gene Ontology Biological Process
- Golgi localization [IDA]
- Golgi reassembly [IMP]
- apoptotic process [IBA]
- establishment of Golgi localization [IMP]
- intracellular signal transduction [IBA]
- intrinsic apoptotic signaling pathway in response to hydrogen peroxide [IGI]
- positive regulation of stress-activated MAPK cascade [IDA]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- regulation of cell differentiation [IBA]
- response to hydrogen peroxide [IDA]
- response to oxidative stress [TAS]
- signal transduction [TAS]
- signal transduction by phosphorylation [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MOB4
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]
Throughput
- High Throughput
Ontology Terms
- hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]
Additional Notes
- exogenous expression of bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STK25 MOB4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1802 | BioGRID | 3480482 | |
| MOB4 STK25 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| STK25 MOB4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| MOB4 STK25 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3270713 | |
| MOB4 STK25 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3561962 |
Curated By
- BioGRID