PRKACB
Gene Ontology Biological Process
- activation of phospholipase C activity [TAS]
- activation of protein kinase A activity [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [TAS]
- blood coagulation [TAS]
- carbohydrate metabolic process [TAS]
- cellular response to glucagon stimulus [TAS]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- protein phosphorylation [IDA]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- transmembrane transport [TAS]
- triglyceride catabolic process [TAS]
- water transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HSP90AA1
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]
Additional Notes
- exogenous expression of bait
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
HSP90AA1 PRKACB | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | Low | - | BioGRID | - |
Curated By
- BioGRID