BAIT
ADRBK1
BARK1, BETA-ARK1, GRK2
adrenergic, beta, receptor kinase 1
GO Process (15)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- G-protein coupled acetylcholine receptor signaling pathway [ISS]
- activation of phospholipase C activity [TAS]
- cardiac muscle contraction [IMP]
- desensitization of G-protein coupled receptor protein signaling pathway [ISS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- negative regulation of striated muscle contraction [IMP]
- negative regulation of the force of heart contraction by chemical signal [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-serine phosphorylation [ISS]
- positive regulation of catecholamine secretion [ISS]
- receptor internalization [IDA]
- signal transduction [TAS]
- tachykinin receptor signaling pathway [IDA]
Gene Ontology Molecular Function
Homo sapiens
PREY
HSP90AA1
EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
heat shock protein 90kDa alpha (cytosolic), class A member 1
GO Process (16)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]
Nat. Methods Apr. 01, 2013; 10(4);307-14 [Pubmed: 23455922]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID