An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.

Varjosalo M, Sacco R, Stukalov A, van Drogen A, Planyavsky M, Hauri S, Aebersold R, Bennett KL, Colinge J, Gstaiger M, Superti-Furga G

The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]

Nat. Methods Apr. 01, 2013; 10(4);307-14 [Pubmed: 23455922]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]

Additional Notes

exogenous expression of bait

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CSNK1E WDR5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Buljan M (2020)	High	986	BioGRID	3482318

Curated By

BioGRID

Interaction Summary

CSNK1E

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]protein kinase activity [ISS]protein serine/threonine kinase activity [IDA, TAS]

Gene Ontology Cellular Component

WDR5