BAIT
SMAD6
AOVD2, HsT17432, MADH6, MADH7
SMAD family member 6
GO Process (13)
GO Function (12)
GO Component (4)
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- cell-substrate adhesion [IMP]
- fat cell differentiation [IDA]
- immune response [IMP]
- intracellular signal transduction [IDA, IMP]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of SMAD protein complex assembly [IDA]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA, IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP]
- response to laminar fluid shear stress [IEP]
- zygotic specification of dorsal/ventral axis [IMP]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
Homo sapiens
PREY
NEDD4L
NEDD4-2, NEDD4.2, RSP5, hNEDD4-2
neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
GO Process (33)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- cellular sodium ion homeostasis [NAS]
- excretion [NAS]
- gene expression [TAS]
- ion transmembrane transport [TAS]
- negative regulation of potassium ion transmembrane transport [IDA]
- negative regulation of potassium ion transmembrane transporter activity [IDA]
- negative regulation of protein localization to cell surface [IDA]
- negative regulation of sodium ion transmembrane transport [IDA]
- negative regulation of sodium ion transmembrane transporter activity [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- positive regulation of caveolin-mediated endocytosis [ISS]
- positive regulation of endocytosis [NAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K48-linked ubiquitination [IDA]
- protein ubiquitination [IDA, NAS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA, IDA]
- regulation of ion transmembrane transport [IDA]
- regulation of membrane depolarization [IDA]
- regulation of membrane potential [IDA]
- regulation of membrane repolarization [IDA]
- regulation of potassium ion transmembrane transporter activity [IDA]
- regulation of protein catabolic process [NAS]
- response to metal ion [IDA]
- sodium ion transport [NAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- transmembrane transport [TAS]
- ventricular cardiac muscle cell action potential [ISS]
- viral life cycle [TAS]
- viral process [TAS]
- water homeostasis [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling.
Protein kinase ALK3/BMPR1A mediates bone morphogenetic protein (BMP) signalling through phosphorylation and activation of SMADs 1/5/8. SMAD6, a transcriptional target of BMP, negatively regulates the BMP pathway by recruiting E3 ubiquitin ligases and targeting ALK3 for ubiquitin-mediated degradation. Here, we identify a deubiquitylating enzyme USP15 as an interactor of SMAD6 and ALK3. We show that USP15 enhances BMP-induced phosphorylation of ... [more]
Open Biol May. 01, 2014; 4(5); [Pubmed: 24850914]
Throughput
- Low Throughput
Curated By
- BioGRID