BAIT

PEP5

END1, VAM1, VPL9, VPS11, VPT11, tethering complex subunit PEP5, L000001376, L000002957, YMR231W
Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitylation and degradation of excess histones
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

TOR1

DRR1, phosphatidylinositol kinase-related protein kinase TOR1, L000002322, YJR066W
PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Endolysosomal Membrane Trafficking Complexes Drive Nutrient-Dependent TORC1 Signaling To Control Cell Growth in Saccharomyces cerevisiae.

Kingsbury JM, Dabas Sen N, Maeda T, Heitman J, Cardenas ME

The rapamycin-sensitive and endomembrane-associated TORC1 pathway controls cell growth in response to nutrients in eukaryotes. Mutations in Class C Vps (Vps-C) complexes are synthetically lethal with tor1 mutations and confer rapamycin hypersensitivity in Saccharomyces cerevisiae, suggesting a role for these complexes in TORC1 signaling. Vps-C complexes are required for vesicular trafficking and fusion, and comprise four distinct complexes; HOPS and ... [more]

Genetics Feb. 10, 2014; 0(0); [Pubmed: 24514902]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: metabolism and growth (APO:0000094)

Additional Notes

  • TOR1-LM mutant suppressed the single mutant phenotypes: both sensitivity to rapamycin and recovery from rapamycin; Fig 2B

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TOR1 PEP5
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
239379

Curated By

  • BioGRID