TRIM3
Gene Ontology Biological Process
Gene Ontology Molecular Function
CDKN1A
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [ISO]
- G1/S transition of mitotic cell cycle [ISO]
- G2/M transition of mitotic cell cycle [ISO]
- cell cycle arrest [IDA, ISO]
- cellular response to DNA damage stimulus [IMP, ISO]
- cellular response to extracellular stimulus [ISO]
- cellular response to ionizing radiation [ISO]
- cellular senescence [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- mitotic cell cycle arrest [IDA]
- negative regulation of G1/S transition of mitotic cell cycle [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of gene expression [IMP]
- negative regulation of phosphorylation [ISO]
- positive regulation of B cell proliferation [IGI]
- positive regulation of fibroblast proliferation [ISO]
- positive regulation of programmed cell death [IMP]
- positive regulation of protein kinase activity [ISO]
- positive regulation of reactive oxygen species metabolic process [ISO]
- protein phosphorylation [ISO]
- regulation of DNA biosynthetic process [IMP]
- regulation of cell cycle [IDA]
- regulation of cyclin-dependent protein serine/threonine kinase activity [IMP]
- regulation of mitotic cell cycle [IMP]
- regulation of protein import into nucleus, translocation [IMP]
- response to UV [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
TRIM3, a tumor suppressor linked to regulation of p21(Waf1/Cip1.).
The TRIM family of genes is largely studied because of their roles in development, differentiation and host cell antiviral defenses; however, roles in cancer biology are emerging. Loss of heterozygosity of the TRIM3 locus in ∼20% of human glioblastomas raised the possibility that this NHL-domain containing member of the TRIM gene family might be a mammalian tumor suppressor. Consistent with ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRIM3 CDKN1A | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID