PREY

LRR1

4-1BBLRR, LRR-1, PPIL5
leucine rich repeat protein 1
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Novel Cul3 binding proteins function to remodel E3 ligase complexes.

Wimuttisuk W, West M, Davidge B, Yu K, Salomon A, Singer JD

Cullins belong to a family of scaffold proteins that assemble multi-subunit ubiquitin ligase complexes to recruit protein substrates for ubiquitination via unique sets of substrate adaptor, such as Skp1 or Elongin B, and a substrate-binding protein with a conserved protein-protein interacting domain, such as leucine-rich repeats (LRR), a WD40 domain, or a zinc-finger domain. In the case of the Cullin3 ... [more]

BMC Cell Biol. Jul. 12, 2014; 15(1);28 [Pubmed: 25011449]

Throughput

  • Low Throughput

Curated By

  • BioGRID