BAIT
ZW10
HZW10, KNTC1AP
zw10 kinetochore protein
GO Process (11)
GO Function (2)
GO Component (8)
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IMP]
- Golgi organization [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- meiotic nuclear division [TAS]
- mitotic cell cycle [TAS]
- mitotic cell cycle checkpoint [IDA]
- mitotic metaphase plate congression [IMP]
- mitotic sister chromatid segregation [IDA]
- protein complex assembly [IDA]
- protein localization to kinetochore [IMP]
- regulation of exit from mitosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ROCK2
ROCK-II
Rho-associated, coiled-coil containing protein kinase 2
GO Process (14)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- axon guidance [TAS]
- centrosome duplication [IMP]
- cytokinesis [NAS]
- negative regulation of angiogenesis [IMP]
- protein phosphorylation [IDA]
- regulation of actin cytoskeleton organization [TAS]
- regulation of cell adhesion [TAS]
- regulation of cell motility [TAS]
- regulation of circadian rhythm [ISS]
- regulation of establishment of cell polarity [TAS]
- regulation of focal adhesion assembly [TAS]
- regulation of keratinocyte differentiation [IMP]
- regulation of stress fiber assembly [TAS]
- smooth muscle contraction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
A high-throughput approach for measuring temporal changes in the interactome.
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use ... [more]
Nat. Methods Sep. 01, 2012; 9(9);907-9 [Pubmed: 22863883]
Throughput
- High Throughput
Ontology Terms
- cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Curated By
- BioGRID