An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

NF-ÎºB-inducing Kinase (NIK) Contains an Amino Terminal Inhibitor of Apoptosis (IAP) Binding Motif (IBM) that Potentiates NIK Degradation by c-IAP1.

Lee S, Challa-Malladi M, Bratton SB, Wright CW

Activation of the noncanonical NF-ÎºB pathway hinges on the stability of the NF-ÎºB-inducing kinase (NIK), which is kept at low levels basally by a protein complex consisting of the E3 ubiquitin ligases cellular inhibitor of apoptosis 1 and 2 (c-IAP1/2) proteins and the tumor necrosis factor receptor associated factors 2 and 3 (TRAF2/3). NIK is brought into close proximity to ... [more]

J. Biol. Chem. Sep. 22, 2014; 0(0); [Pubmed: 25246529]

Throughput

Low Throughput

Additional Notes

Figure 2

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MAP3K14 TRAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Buljan M (2020)	High	578	BioGRID	3486180
MAP3K14 TRAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hubel P (2019)	High	-	BioGRID	-
MAP3K14 TRAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9999	BioGRID	2226541
MAP3K14 TRAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9997	BioGRID	3080938
MAP3K14 TRAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	0.9995	BioGRID	3283961
MAP3K14 TRAF3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kennedy SA (2020)	High	-	BioGRID	-
MAP3K14 TRAF3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Choudhary S (2013)	Low	-	BioGRID	-
MAP3K14 TRAF3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liao G (2004)	Low	-	BioGRID	-
TRAF3 MAP3K14	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sanjo H (2010)	Low	-	BioGRID	678992
MAP3K14 TRAF3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sanjo H (2010)	Low	-	BioGRID	678998
TRAF3 MAP3K14	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Song HY (1997)	Low	-	BioGRID	-
TRAF3 MAP3K14	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhang B (2020)	Low	-	BioGRID	-
MAP3K14 TRAF3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhang B (2020)	Low	-	BioGRID	-
MAP3K14 TRAF3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Liao G (2004)	Low	-	BioGRID	-
MAP3K14 TRAF3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Luftig MA (2001)	Low	-	BioGRID	-
MAP3K14 TRAF3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hu WH (2004)	Low	-	BioGRID	-
MAP3K14 TRAF3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2694069

Curated By

BioGRID

Interaction Summary

MAP3K14

Gene Ontology Biological Process

Gene Ontology Molecular Function

MAP kinase kinase activity [IBA]NF-kappaB-inducing kinase activity [ISS]protein binding [IPI]protein kinase activity [IDA]receptor signaling protein serine/threonine kinase activity [IBA]

Gene Ontology Cellular Component

TRAF3

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]signal transducer activity [TAS]thioesterase binding [IPI]tumor necrosis factor receptor binding [IPI]ubiquitin protein ligase binding [IPI]