Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interaction of human leukotriene C4 synthase and microsomal glutathione transferase in vivo.

Surapureddi S, Morgenstern R, Soederstroem M, Hammarstroem S

Microsomal glutathione transferase (mGT) specifically binds leukotriene C4 synthase in the presence of Mg2+ ion (Soederstroem et al., Protein Expression and Purification (1995) 6, 352-356). To investigate if this interaction occurs in vivo we screened a human lung cDNA library with a bait vector encoding human mGT in the yeast two-hybrid system. One of the five positive clones obtained encoded ... [more]

Biochem. Biophys. Res. Commun. Dec. 13, 1996; 229(2);388-95 [Pubmed: 8954909]

Throughput

  • High Throughput

Curated By

  • BioGRID