BAIT

DFR

DIHYDROFLAVONOL 4-REDUCTASE, DIHYDROKAEMPFEROL 4-REDUCTASE, M318, MJB21.18, MJB21_18, TT3, AT5G42800
dihydroflavonol-4-reductase
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

TT4

ATCHS, CHALCONE SYNTHASE, CHALCONE/STILBENE SYNTHASE, CHS, MAC12.28, MAC12_28, NARINGENIN-CHALCONE SYNTHASE, TRANSPARENT TESTA 4, AT5G13930
chalcone synthase
Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Foerster resonance energy transfer demonstrates a flavonoid metabolon in living plant cells that displays competitive interactions between enzymes.

Crosby KC, Pietraszewska-Bogiel A, Gadella TW, Winkel BS

We have used Foerster resonance energy transfer detected by fluorescence lifetime imaging microscopy (FLIM-FRET) to provide the first evidence from living plants cells for the existence of a flavonoid metabolon. The distribution of flux within this system may be regulated by the direct competition of enzymes that catalyze key branch-point reactions, flavonol synthase 1 and dihydroflavonol 4-reductase, for association with ... [more]

FEBS Lett. Jul. 21, 2011; 585(14);2193-8 [Pubmed: 21669202]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TT4 DFR
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BAR
-

Curated By

  • BioGRID