BAIT

TT4

ATCHS, CHALCONE SYNTHASE, CHALCONE/STILBENE SYNTHASE, CHS, MAC12.28, MAC12_28, NARINGENIN-CHALCONE SYNTHASE, TRANSPARENT TESTA 4, AT5G13930
chalcone synthase
Arabidopsis thaliana (Columbia)
PREY

DFR

DIHYDROFLAVONOL 4-REDUCTASE, DIHYDROKAEMPFEROL 4-REDUCTASE, M318, MJB21.18, MJB21_18, TT3, AT5G42800
dihydroflavonol-4-reductase
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interactions among enzymes of the Arabidopsis flavonoid biosynthetic pathway.

Burbulis IE, Winkel-Shirley B

Flavonoids are secondary metabolites derived from phenylalanine and acetate metabolism that perform a variety of essential functions in higher plants. Studies over the past 30 years have supported a model in which flavonoid metabolism is catalyzed by an enzyme complex localized to the endoplasmic reticulum [Hrazdina, G. & Wagner, G. J. (1985) Arch. Biochem. Biophys. 237, 88-100]. To test this ... [more]

Proc. Natl. Acad. Sci. U.S.A. Oct. 26, 1999; 96(22);12929-34 [Pubmed: 10536025]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DFR TT4
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
-

Curated By