An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.

Lleres D, Denegri M, Biggiogera M, Ajuh P, Lamond AI

Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required ... [more]

EMBO Rep. Jun. 01, 2010; 11(6);445-51 [Pubmed: 20467437]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDC5L EXOC7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Camargo LM (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDC5L

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [NAS]WD40-repeat domain binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

EXOC7