Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.

Floyd BJ, Wilkerson EM, Veling MT, Minogue CE, Xia C, Beebe ET, Wrobel RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JP, Coon JJ, Pagliarini DJ

Mitochondria are essential for numerous cellular processes, yet hundreds of their proteins lack robust functional annotation. To reveal functions for these proteins (termed MXPs), we assessed condition-specific protein-protein interactions for 50 select MXPs using affinity enrichment mass spectrometry. Our data connect MXPs to diverse mitochondrial processes, including multiple aspects of respiratory chain function. Building upon these observations, we validated C17orf89 ... [more]

Mol. Cell Aug. 03, 2016; 0(0); [Pubmed: 27499296]

Quantitative Score

  • 96.8 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • The scores represent percentile of the interaction (out of all interactions) based on the CompPASS algorithm.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CLPB NDUFS3
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High0.94BioGRID
2849656

Curated By

  • BioGRID