Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A High-Density Human Mitochondrial Proximity Interaction Network.

Antonicka H, Lin ZY, Janer A, Aaltonen MJ, Weraarpachai W, Gingras AC, Shoubridge EA

We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously annotated as mitochondrial, nearly half of the mitochondrial proteome defined by Mitocarta 2.0. Bait-bait analysis showed a clear separation of ... [more]

Cell Metab. Sep. 01, 2020; 32(3);479-497.e9 [Pubmed: 32877691]

Quantitative Score

  • 0.94 [Saint Score]

Throughput

  • High Throughput

Additional Notes

  • interaction assayed using BioID
  • interactions were considered high confidence if they had a Bayesian False Discovery Rate of 1% or less
  • the Saint Score for the interaction (or the maximum of any bait-prey combinations that had multiple scores) is shown

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NDUFS3 CLPB
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High96.8BioGRID
1525107

Curated By

  • BioGRID