BAIT

S100A11

HEL-S-43, MLN70, S100C
S100 calcium binding protein A11
Homo sapiens
PREY

PLA2G4A

PLA2G4, cPLA2-alpha
phospholipase A2, group IVA (cytosolic, calcium-dependent)
Alzheimer's Disease Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Truncation of annexin A1 is a regulatory lever for linking epidermal growth factor signaling with cytosolic phospholipase A2 in normal and malignant squamous epithelial cells.

Sakaguchi M, Murata H, Sonegawa H, Sakaguchi Y, Futami J, Kitazoe M, Yamada H, Huh NH

Regulation of cell growth and apoptosis is one of the pleiotropic functions of annexin A1 (ANXA1). Although previous reports on the overexpression of ANXA1 in many human cancers and on growth suppression and/or induction of apoptosis by ANXA1 may indicate the tumor-suppressive nature of ANXA1, molecular mechanisms of the function of ANXA1 remain largely unknown. Here we provide evidence that ... [more]

J. Biol. Chem. Dec. 07, 2007; 282(49);35679-86 [Pubmed: 17932043]

Throughput

  • Low Throughput

Additional Notes

  • Figure 2

Curated By

  • BioGRID