BAIT

SWI4

ART1, SBF complex DNA-binding subunit SWI4, L000000124, L000002252, YER111C

DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]

positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

SBF transcription complex [IDA, IMP, IPI]

nuclear chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

PKC1

CLY15, CLY5, CLY7, HPO2, STT1, protein kinase C, L000001446, L000000362, S000029091, YBL105C

Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)

Kinome Project (Sc)

GO Process (8)

GO Function (1)

GO Component (6)

Gene Ontology Biological Process

actin filament organization [IGI]

cytoplasmic mRNA processing body assembly [IMP]

intracellular signal transduction [IMP]

peroxisome degradation [IMP]

protein phosphorylation [IDA]

regulation of fungal-type cell wall organization [IMP]

regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]

signal transduction [IMP]

Gene Ontology Molecular Function

protein kinase C activity [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytoskeleton [IDA]

nucleus [IDA]

plasma membrane [IDA]

prospore septin filament array [IDA]

site of polarized growth [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Coordinated regulation of gene expression by the cell cycle transcription factor Swi4 and the protein kinase C MAP kinase pathway for yeast cell integrity.

Igual JC, Johnson AL, Johnston LH

Specific transcription in late G1, mediated by the transcription factors SBF (Swi4p-Swi6p) and MBF (Mbp1p-Swi6p), is crucial for cell cycle progression in budding yeast. In order to better understand the G1/S transition, we initiated a search for conditional mutations synthetic lethal with swi4delta. One of the isolated mutants, rsf8swi4delta, showed a growth defect due to cell lysis. rsf8 is allelic ... [more]

EMBO J. Sep. 16, 1996; 15(18);5001-13 [Pubmed: 8890173]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SWI4 PKC1	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Gray JV (1997)	Low	-	BioGRID	155025
SWI4 PKC1	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Kaeberlein M (2002)	Low	-	BioGRID	657773
SWI4 PKC1	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Queralt E (2005)	Low	-	BioGRID	429927
PKC1 SWI4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1259	BioGRID	1959517
PKC1 SWI4	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Krause SA (2009)	Low	-	BioGRID	343617
PKC1 SWI4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Igual JC (1996)	Low	-	BioGRID	158478
PKC1 SWI4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Igual JC (1997)	Low	-	BioGRID	158476
SWI4 PKC1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Igual JC (1997)	Low	-	BioGRID	158477
PKC1 SWI4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Krause SA (2008)	Low	-	BioGRID	327817
PKC1 SWI4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Madden K (1997)	Low	-	BioGRID	163601

Curated By

BioGRID

Interaction Summary

SWI4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

PKC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein kinase C activity [IDA]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

Coordinated regulation of gene expression by the cell cycle transcription factor Swi4 and the protein kinase C MAP kinase pathway for yeast cell integrity.

Throughput

Ontology Terms

Related interactions

Curated By

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]