BAIT

RAD27

ERC11, FEN1, RTH1, multifunctional nuclease RAD27, L000002742, L000000565, YKL113C

5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia

GO Process (6)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

DNA replication, removal of RNA primer [IDA]

base-excision repair, base-free sugar-phosphate removal [IGI, IMP]

double-strand break repair via nonhomologous end joining [IDA]

gene conversion at mating-type locus, DNA repair synthesis [IMP]

maintenance of DNA trinucleotide repeats [IGI, IMP]

replicative cell aging [IMP]

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]
5'-flap endonuclease activity [IDA]

Gene Ontology Cellular Component

cytosol [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RAD51

MUT5, recombinase RAD51, L000001571, YER095W

Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein

Kinome Project (Sc)

GO Process (7)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

DNA recombination [IDA]

double-strand break repair via single-strand annealing [IMP]

heteroduplex formation [IDA]

meiotic joint molecule formation [IMP]

reciprocal meiotic recombination [IMP]

strand invasion [IDA]

telomere maintenance via recombination [IMP]

Gene Ontology Molecular Function

DNA-dependent ATPase activity [IDA]
recombinase activity [IDA]
single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

condensed nuclear chromosome [IDA]

nuclear chromosome [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Genetic network interactions among replication, repair and nuclear pore deficiencies in yeast.

Loeillet S, Palancade B, Cartron M, Thierry A, Richard GF, Dujon B, Doye V, Nicolas A

The yeast RAD27 gene encodes a functional homolog of the mammalian FEN1 protein, a structure-specific endo/exonuclease which plays an important role in DNA replication and repair. Previous genetic interaction studies, including a synthetic genetic array (SGA) analysis, showed that the survival of rad27Delta cells requires several DNA metabolic processes, in particular those mediated by all members of the Rad52-dependent recombinational ... [more]

DNA Repair (Amst.) Apr. 04, 2005; 4(4);459-68 [Pubmed: 15725626]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Collins SR (2007)	High	-7.8658	BioGRID	215160
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3931	BioGRID	394093
RAD51 RAD27	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3931	BioGRID	375683
RAD51 RAD27	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.324	BioGRID	2109044
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4147	BioGRID	2143623
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Fiedler D (2009)	High	-7.7975	BioGRID	323103
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Surma MA (2013)	High	-4.4355	BioGRID	901433
RAD51 RAD27	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Gary R (1999)	Low	-	BioGRID	427835
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Debrauwere H (2001)	Low	-	BioGRID	657076
RAD51 RAD27	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Pan X (2006)	High	-	BioGRID	456249
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Pan X (2006)	High	-	BioGRID	455070
RAD51 RAD27	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Symington LS (1998)	Low	-	BioGRID	158601
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tishkoff DX (1997)	Low	-	BioGRID	158592
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2001)	High	-	BioGRID	109272
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	450128

Curated By

BioGRID

Interaction Summary

RAD27

Gene Ontology Biological Process

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]5'-flap endonuclease activity [IDA]

Gene Ontology Cellular Component

RAD51

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-dependent ATPase activity [IDA]recombinase activity [IDA]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

Genetic network interactions among replication, repair and nuclear pore deficiencies in yeast.

Throughput

Ontology Terms

Related interactions

Curated By

5'-3' exonuclease activity [IDA]
5'-flap endonuclease activity [IDA]

DNA-dependent ATPase activity [IDA]
recombinase activity [IDA]
single-stranded DNA binding [IDA]