BAIT

RAD51

MUT5, recombinase RAD51, L000001571, YER095W

Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein

Kinome Project (Sc)

GO Process (7)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

DNA recombination [IDA]

double-strand break repair via single-strand annealing [IMP]

heteroduplex formation [IDA]

meiotic joint molecule formation [IMP]

reciprocal meiotic recombination [IMP]

strand invasion [IDA]

telomere maintenance via recombination [IMP]

Gene Ontology Molecular Function

DNA-dependent ATPase activity [IDA]
recombinase activity [IDA]
single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

condensed nuclear chromosome [IDA]

nuclear chromosome [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RAD27

ERC11, FEN1, RTH1, multifunctional nuclease RAD27, L000002742, L000000565, YKL113C

5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia

GO Process (6)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

DNA replication, removal of RNA primer [IDA]

base-excision repair, base-free sugar-phosphate removal [IGI, IMP]

double-strand break repair via nonhomologous end joining [IDA]

gene conversion at mating-type locus, DNA repair synthesis [IMP]

maintenance of DNA trinucleotide repeats [IGI, IMP]

replicative cell aging [IMP]

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]
5'-flap endonuclease activity [IDA]

Gene Ontology Cellular Component

cytosol [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.324 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: rad51 - rad27 [SGA score = -0.3240, P-value = 5.787E-12]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Collins SR (2007)	High	-7.8658	BioGRID	215160
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3931	BioGRID	394093
RAD51 RAD27	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3931	BioGRID	375683
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4147	BioGRID	2143623
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Fiedler D (2009)	High	-7.7975	BioGRID	323103
RAD27 RAD51	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Surma MA (2013)	High	-4.4355	BioGRID	901433
RAD51 RAD27	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Gary R (1999)	Low	-	BioGRID	427835
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Debrauwere H (2001)	Low	-	BioGRID	657076
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Loeillet S (2005)	Low	-	BioGRID	166743
RAD51 RAD27	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Pan X (2006)	High	-	BioGRID	456249
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Pan X (2006)	High	-	BioGRID	455070
RAD51 RAD27	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Symington LS (1998)	Low	-	BioGRID	158601
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tishkoff DX (1997)	Low	-	BioGRID	158592
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2001)	High	-	BioGRID	109272
RAD27 RAD51	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	450128

Curated By

BioGRID

Interaction Summary

RAD51

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-dependent ATPase activity [IDA]recombinase activity [IDA]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

RAD27

Gene Ontology Biological Process

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]5'-flap endonuclease activity [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

DNA-dependent ATPase activity [IDA]
recombinase activity [IDA]
single-stranded DNA binding [IDA]

5'-3' exonuclease activity [IDA]
5'-flap endonuclease activity [IDA]