CLB2
Gene Ontology Biological Process
Gene Ontology Molecular Function
FKH2
Gene Ontology Biological Process
- chromatin remodeling [IGI, IMP]
- mitochondrion organization [IBA]
- negative regulation of chromatin silencing at silent mating-type cassette [IGI, IMP]
- negative regulation of pseudohyphal growth [IGI, IMP]
- negative regulation of transcription involved in G1/S transition of mitotic cell cycle [IGI]
- negative regulation of transcription involved in G2/M transition of mitotic cell cycle [IGI]
- positive regulation of DNA-dependent DNA replication initiation [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IGI, IMP]
- positive regulation of transcription involved in G2/M transition of mitotic cell cycle [IGI, IMP]
- regulation of sequence-specific DNA binding transcription factor activity [IBA]
Gene Ontology Molecular Function- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA]
- RNA polymerase II transcription factor binding [IDA, IMP]
- RNA polymerase II transcription factor binding transcription factor activity [IDA, IGI, IMP]
- double-stranded DNA binding [IBA]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IBA]
- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA]
- RNA polymerase II transcription factor binding [IDA, IMP]
- RNA polymerase II transcription factor binding transcription factor activity [IDA, IGI, IMP]
- double-stranded DNA binding [IBA]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IBA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Decoding common and divergent cellular functions of the domains of forkhead transcription factors Fkh1 and Fkh2.
Forkhead transcription factors play a key role in embryonic patterning during development. In Saccharomyces cerevisiae, two forkhead transcription factors, Fkh1 and Fkh2, regulate the transcription of CLB2 cluster genes important for mitosis. Fkh1 reduces, whereas Fkh2 elevates, the transcription of CLB2 cluster genes. However, the mechanism for this observation remains unclear. Fkh1 and Fkh2 each contain a forkhead domain (DNA-binding ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FKH2 CLB2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| FKH2 CLB2 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 154290 | |
| CLB2 FKH2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -4.5991 | BioGRID | 325161 |
Curated By
- BioGRID