An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Group IVA Cytosolic Phospholipase A2 Regulates the G2-to-M Transition by Modulating the Activity of Tumor Suppressor SIRT2.

Movahedi Naini S, Sheridan AM, Force T, Shah JV, Bonventre JV

The G2-to-M transition (or prophase) checkpoint of the cell cycle is a critical regulator of mitotic entry. SIRT2, a tumor suppressor gene, contributes to the control of this checkpoint by blocking mitotic entry under cellular stress. However, the mechanism underlying both SIRT2 activation and regulation of the G2-to-M transition remains largely unknown. Here, we report the formation of a multiprotein ... [more]

Mol. Cell. Biol. Nov. 01, 2015; 35(21);3768-84 [Pubmed: 26303530]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PLA2G4A

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [IDA]calcium-dependent phospholipase A2 activity [EXP]calcium-dependent phospholipid binding [IDA]phospholipase A2 activity [IDA, NAS, TAS]

Gene Ontology Cellular Component

CCNA2