BRAF
Gene Ontology Biological Process
- activation of MAPKK activity [TAS]
- cellular response to calcium ion [IDA]
- fibroblast growth factor receptor signaling pathway [TAS]
- negative regulation of apoptotic process [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- organ morphogenesis [TAS]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- protein phosphorylation [IDA]
- small GTPase mediated signal transduction [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
YWHAG
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- apoptotic process [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- negative regulation of protein kinase activity [NAS]
- negative regulation of protein serine/threonine kinase activity [NAS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- regulation of neuron differentiation [IMP]
- regulation of signal transduction [NAS]
- regulation of synaptic plasticity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Architecture of the human interactome defines protein communities and disease networks.
The physiology of a cell can be viewed as the product of thousands of proteins acting in concert to shape the cellular response. Coordination is achieved in part through networks of protein-protein interactions that assemble functionally related proteins into complexes, organelles, and signal transduction pathways. Understanding the architecture of the human proteome has the potential to inform cellular, structural, and ... [more]
Quantitative Score
- 0.996036294 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 2.0 HEK 293T cells CompPASS score = 0.996036294, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 3.0 (PMID: 33961781) and may be re-scored from BioPlex 1.0 (PMID: 26186194). Only scores from within BioPlex 2.0 (PMID: 28514442) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BRAF YWHAG | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 480.8571 | BioGRID | 3481416 | |
| YWHAG BRAF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3376042 | |
| BRAF YWHAG | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| YWHAG BRAF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.535 | BioGRID | 241582 | |
| BRAF YWHAG | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| YWHAG BRAF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3121675 | |
| YWHAG BRAF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3236364 | |
| YWHAG BRAF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| BRAF YWHAG | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| BRAF YWHAG | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2469662 | |
| YWHAG BRAF | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3532109 | |
| BRAF YWHAG | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | High | - | BioGRID | 2640592 | |
| YWHAG BRAF | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3534781 |
Curated By
- BioGRID