EMC
Gene Ontology Biological Process
- R3/R4 cell differentiation [IMP]
- R7 cell differentiation [IMP]
- R8 cell fate commitment [NAS]
- brain morphogenesis [IMP]
- cell proliferation [IGI, IMP]
- chaeta morphogenesis [TAS]
- chorion-containing eggshell pattern formation [IMP]
- compound eye cone cell differentiation [IMP]
- dorsal appendage formation [IMP]
- dorsal closure [IMP]
- head involution [IMP]
- imaginal disc-derived wing morphogenesis [IGI, IMP]
- imaginal disc-derived wing vein morphogenesis [IGI, IMP]
- inter-male aggressive behavior [IMP]
- lateral inhibition [IMP]
- locomotion involved in locomotory behavior [IMP]
- midgut development [IMP]
- negative regulation of DNA binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- peripheral nervous system development [IMP]
- photoreceptor cell fate commitment [IMP]
- progression of morphogenetic furrow involved in compound eye morphogenesis [IMP]
- sex determination [IGI]
- sex determination, primary response to X:A ratio [TAS]
- spermatid development [IMP]
- startle response [IMP]
Gene Ontology Molecular Function
DA
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A basic-helix-loop-helix protein expressed in precursors of Drosophila longitudinal visceral muscles.
Basic-helix-loop-helix (bHLH) transcription factors are involved in the control of many developmental processes in vertebrates and invertebrates. The HLH domain mediates formation of homo- or heterodimers. We have taken advantage of these dimerisation properties to identify a novel Drosophila HLH protein using the yeast two-hybrid system. Expression of bHLH54F at the blastoderm stage is restricted to a small subpopulation of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EMC DA | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | FlyBase | - | |
| EMC DA | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 2773605 | |
| EMC DA | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| EMC DA | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| EMC DA | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| EMC DA | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - |