BAIT

EMC

0094/26, 0203/10, 0587/01, 0977/09, Ach, CG1007, Dm0688, Dmel\CG1007, bHLHb28, gov, l(3)04322, l(3)05592, l(3)61Da, l(3)j4E11, ms(3)61CD, Dmel_CG1007
extra macrochaetae
Drosophila melanogaster
PREY

DA

5102, CG5102, Dmel\CG5102, bHLHb22, l(2)31Ea, Dmel_CG5102
daughterless
GO Process (0)
GO Function (0)
GO Component (0)
Drosophila melanogaster

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

A Comprehensive Drosophila melanogaster Transcription Factor Interactome.

Shokri L, Inukai S, Hafner A, Weinand K, Hens K, Vedenko A, Gisselbrecht SS, Dainese R, Bischof J, Furger E, Feuz JD, Basler K, Deplancke B, Bulyk ML

Combinatorial interactions among transcription factors (TFs) play essential roles in generating gene expression specificity and diversity in metazoans. Using yeast 2-hybrid (Y2H) assays on nearly all sequence-specific Drosophila TFs, we identified 1,983 protein-protein interactions (PPIs), more than doubling the number of currently known PPIs among Drosophila TFs. For quality assessment, we validated a subset of our interactions using MITOMI and ... [more]

Cell Rep Dec. 16, 2018; 27(3);955-970.e7 [Pubmed: 30995488]

Throughput

  • Low Throughput

Additional Notes

  • BiFC

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EMC DA
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-FlyBase
-
EMC DA
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-FlyBase
-
EMC DA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-FlyBase
-
EMC DA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-FlyBase
-
EMC DA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
EMC DA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-FlyBase
-

Curated By

  • BioGRID