BAIT
FUS3
DAC2, mitogen-activated serine/threonine-protein kinase FUS3, L000000655, YBL016W
Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis
GO Process (8)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- cell cycle arrest [IMP]
- invasive growth in response to glucose limitation [IMP]
- negative regulation of MAPK cascade [IPI]
- negative regulation of transposition, RNA-mediated [IMP]
- pheromone-dependent signal transduction involved in conjugation with cellular fusion [IDA]
- positive regulation of protein export from nucleus [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MSH2
PMS5, mismatch repair ATPase MSH2, L000001190, YOL090W
Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
GO Process (11)
GO Function (11)
GO Component (4)
Gene Ontology Biological Process
- DNA recombination [IMP]
- chromatin silencing at silent mating-type cassette [IGI]
- interstrand cross-link repair [IGI]
- maintenance of DNA repeat elements [IBA]
- meiotic gene conversion [IMP]
- meiotic mismatch repair [IMP]
- mismatch repair [IMP]
- mitotic recombination [IMP]
- negative regulation of reciprocal meiotic recombination [IBA]
- postreplication repair [IBA]
- removal of nonhomologous ends [IGI, IMP]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- DNA insertion or deletion binding [IDA]
- DNA-dependent ATPase activity [IBA]
- Y-form DNA binding [IDA]
- damaged DNA binding [IBA]
- double-strand/single-strand DNA junction binding [IDA]
- four-way junction DNA binding [IDA]
- guanine/thymine mispair binding [IDA]
- heteroduplex DNA loop binding [IDA]
- single base insertion or deletion binding [IDA, IMP]
- ATP binding [IDA]
- ATPase activity [IDA]
- DNA insertion or deletion binding [IDA]
- DNA-dependent ATPase activity [IBA]
- Y-form DNA binding [IDA]
- damaged DNA binding [IBA]
- double-strand/single-strand DNA junction binding [IDA]
- four-way junction DNA binding [IDA]
- guanine/thymine mispair binding [IDA]
- heteroduplex DNA loop binding [IDA]
- single base insertion or deletion binding [IDA, IMP]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A Network of Conserved Synthetic Lethal Interactions for Exploration of Precision Cancer Therapy.
An emerging therapeutic strategy for cancer is to induce selective lethality in a tumor by exploiting interactions between its driving mutations and specific drug targets. Here we use a multi-species approach to develop a resource of synthetic lethal interactions relevant to cancer therapy. First, we screen in yeast ∼169,000 potential interactions among orthologs of human tumor suppressor genes (TSG) and ... [more]
Mol. Cell Aug. 04, 2016; 63(3);514-25 [Pubmed: 27453043]
Quantitative Score
- -2.87 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Untreated conditions. SGA was used to score genetic interactions based on the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID