BAIT

LSM2

SMX5, SNP3, Sm-like protein LSM2, L000003168, YBL026W

Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

GO Process (1)

GO Function (1)

GO Component (7)

Gene Ontology Biological Process

mRNA splicing, via spliceosome [IPI]

Gene Ontology Molecular Function

RNA binding [IDA, IPI]

Gene Ontology Cellular Component

U4/U6 x U5 tri-snRNP complex [IDA]

U6 snRNP [IDA]

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA]

nucleolus [IDA]

nucleus [IDA]

small nucleolar ribonucleoprotein complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SNR6

U6, L000001961

U6 spliceosomal RNA (U6 snRNA); mediates catalysis of pre-mRNA splicing by the spliceosome; interacts with U4; homology to U6 snRNA, interacts with snr14

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities.

Didychuk AL, Montemayor EJ, Carrocci TJ, DeLaitsch AT, Lucarelli SE, Westler WM, Brow DA, Hoskins AA, Butcher SE

U6 small nuclear ribonucleoprotein (snRNP) biogenesis is essential for spliceosome assembly, but not well understood. Here, we report structures of the U6 RNA processing enzyme Usb1 from yeast and a substrate analog bound complex from humans. Unlike the human ortholog, we show that yeast Usb1 has cyclic phosphodiesterase activity that leaves a terminal 3' phosphate which prevents overprocessing. Usb1 processing ... [more]

Nat Commun Dec. 08, 2016; 8(1);497 [Pubmed: 28887445]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LSM2 SNR6	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Roth AJ (2018)	Low	-	BioGRID	3019545
LSM2 SNR6	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Fu X (2022)	Low	-	BioGRID	-
LSM2 SNR6	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Montemayor EJ (2018)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary