BAIT

LSM2

SMX5, SNP3, Sm-like protein LSM2, L000003168, YBL026W

Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

GO Process (1)

GO Function (1)

GO Component (7)

Gene Ontology Biological Process

mRNA splicing, via spliceosome [IPI]

Gene Ontology Molecular Function

RNA binding [IDA, IPI]

Gene Ontology Cellular Component

U4/U6 x U5 tri-snRNP complex [IDA]

U6 snRNP [IDA]

cytoplasm [IDA]

cytoplasmic mRNA processing body [IDA]

nucleolus [IDA]

nucleus [IDA]

small nucleolar ribonucleoprotein complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SNR6

U6, L000001961

U6 spliceosomal RNA (U6 snRNA); mediates catalysis of pre-mRNA splicing by the spliceosome; interacts with U4; homology to U6 snRNA, interacts with snr14

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Defining essential elements and genetic interactions of the yeast Lsm2-8 ring and demonstration that essentiality of Lsm2-8 is bypassed via overexpression of U6 snRNA or the U6 snRNP subunit Prp24.

Roth AJ, Shuman S, Schwer B

A seven-subunit Lsm2-8 protein ring assembles on the U-rich 3' end of the U6 snRNA. A structure-guided mutational analysis of the Saccharomyces cerevisiae Lsm2-8 ring affords new insights to structure-function relations and genetic interactions of the Lsm subunits. Alanine scanning of 39 amino acids comprising the RNA-binding sites or intersubunit interfaces of Lsm2, Lsm3, Lsm4, Lsm5, and Lsm8 identified only ... [more]

RNA Dec. 01, 2017; 24(6);853-864 [Pubmed: 29615482]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: heat sensitivity (APO:0000147)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LSM2 SNR6	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Didychuk AL (2017)	Low	-	BioGRID	-
LSM2 SNR6	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Fu X (2022)	Low	-	BioGRID	-
LSM2 SNR6	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Montemayor EJ (2018)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary