BAIT
RAD51
MUT5, recombinase RAD51, L000001571, YER095W
Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
GO Process (7)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
RFA3
RPA14, RPA3, L000001622, YJL173C
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress
GO Process (14)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
- DNA repair [IMP]
- DNA replication [IMP]
- DNA topological change [IDA]
- DNA unwinding involved in DNA replication [IDA]
- double-strand break repair via homologous recombination [IGI]
- establishment of protein localization [IPI]
- heteroduplex formation [IDA]
- mitotic recombination [IPI]
- nucleotide-excision repair [IDA]
- protein ubiquitination [IPI]
- reciprocal meiotic recombination [IPI]
- telomere maintenance via recombination [IPI]
- telomere maintenance via telomerase [IPI]
- telomere maintenance via telomere lengthening [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament.
RAD54 is an important member of the RAD52 group of genes that carry out recombinational repair of DNA damage in the yeast Saccharomyces cerevisiae. Rad54 protein is a member of the Snf2/Swi2 protein family of DNA-dependent/stimulated ATPases, and its ATPase activity is crucial for Rad54 protein function. Rad54 protein and Rad54-K341R, a mutant protein defective in the Walker A box ... [more]
J. Mol. Biol. Apr. 13, 2001; 307(5);1207-21 [Pubmed: 11292336]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RFA3 RAD51 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2824 | BioGRID | 1993493 |
Curated By
- BioGRID