BAIT

HRR25

KTI14, serine/threonine protein kinase HRR25, L000000810, YPL204W

Protein kinase; involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; phosphorylates the COPII coat; phosphorylates Tif6p which plays a critical role in 60S ribosomal subunit biogenesis; interacts with Sit4p phosphatase; homolog of mammalian casein kinase 1delta (CK1delta)

Kinome Project (Sc)

GO Process (8)

GO Function (2)

GO Component (9)

Gene Ontology Biological Process

DNA repair [IMP]

ER to Golgi vesicle-mediated transport [IMP]

attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation [IMP]

mitotic nuclear division [IMP]

protein phosphorylation [IDA, IMP]

ribosomal large subunit biogenesis [IMP]

ribosomal small subunit biogenesis [IMP]

tRNA wobble uridine modification [IMP]

Gene Ontology Molecular Function

protein kinase activity [IDA]
protein serine/threonine kinase activity [IDA, IMP]

Gene Ontology Cellular Component

cellular bud neck [IDA]

cellular bud tip [IDA]

chromosome, centromeric region [IDA]

cytoplasm [IDA]

monopolin complex [IDA, IMP, IPI]

nucleus [IDA]

peroxisomal importomer complex [IDA]

plasma membrane [IDA]

spindle pole body [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

ESL1

YIL151C

Protein of unknown function; predicted to contain a PINc domain; ESL1 has a paralog, ESL2, that arose from the whole genome duplication

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interaction of CK1Î´ with Î³TuSC ensures proper microtubule assembly and spindle positioning.

Peng Y, Moritz M, Han X, Giddings TH, Lyon A, Kollman J, Winey M, Yates J, Agard DA, Drubin DG, Barnes G

Casein kinase 1Î´ (CK1Î´) family members associate with microtubule-organizing centers (MTOCs) from yeast to humans, but their mitotic roles and targets have yet to be identified. We show here that budding yeast CK1Î´, Hrr25, is a Î³-tubulin small complex (Î³TuSC) binding factor. Moreover, Hrr25's association with Î³TuSC depends on its kinase activity and its noncatalytic central domain. Loss of Hrr25 ... [more]

Mol. Biol. Cell Jul. 01, 2015; 26(13);2505-18 [Pubmed: 25971801]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HRR25 ESL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Dehecq M (2018)	Low	-	BioGRID	-
HRR25 ESL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3614736
HRR25 ESL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Schaefer T (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary