BAIT

HRR25

KTI14, serine/threonine protein kinase HRR25, L000000810, YPL204W

Protein kinase; involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; phosphorylates the COPII coat; phosphorylates Tif6p which plays a critical role in 60S ribosomal subunit biogenesis; interacts with Sit4p phosphatase; homolog of mammalian casein kinase 1delta (CK1delta)

Kinome Project (Sc)

GO Process (8)

GO Function (2)

GO Component (9)

Gene Ontology Biological Process

DNA repair [IMP]

ER to Golgi vesicle-mediated transport [IMP]

attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation [IMP]

mitotic nuclear division [IMP]

protein phosphorylation [IDA, IMP]

ribosomal large subunit biogenesis [IMP]

ribosomal small subunit biogenesis [IMP]

tRNA wobble uridine modification [IMP]

Gene Ontology Molecular Function

protein kinase activity [IDA]
protein serine/threonine kinase activity [IDA, IMP]

Gene Ontology Cellular Component

cellular bud neck [IDA]

cellular bud tip [IDA]

chromosome, centromeric region [IDA]

cytoplasm [IDA]

monopolin complex [IDA, IMP, IPI]

nucleus [IDA]

peroxisomal importomer complex [IDA]

plasma membrane [IDA]

spindle pole body [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

ESL1

YIL151C

Protein of unknown function; predicted to contain a PINc domain; ESL1 has a paralog, ESL2, that arose from the whole genome duplication

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Nonsense-mediated mRNA decay involves two distinct Upf1-bound complexes.

Dehecq M, Decourty L, Namane A, Proux C, Kanaan J, Le Hir H, Jacquier A, Saveanu C

Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA degradation pathway involved in many cellular pathways and crucial for telomere maintenance and embryo development. Core NMD factors Upf1, Upf2 and Upf3 are conserved from yeast to mammals, but a universal NMD model is lacking. We used affinity purification coupled with mass spectrometry and an improved data analysis protocol to characterize the ... [more]

EMBO J. Nov. 02, 2018; 37(21); [Pubmed: 30275269]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HRR25 ESL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3614736
HRR25 ESL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Peng Y (2015)	Low	-	BioGRID	-
HRR25 ESL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Schaefer T (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary