SAE2
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IDA, IMP]
- DNA double-strand break processing [IGI]
- DNA double-strand break processing involved in repair via synthesis-dependent strand annealing [IMP]
- gene conversion at mating-type locus, DNA double-strand break processing [IMP]
- meiotic DNA double-strand break formation [IGI]
- meiotic DNA double-strand break processing [IGI, IMP]
- telomere maintenance [IMP]
- telomeric 3' overhang formation [IMP]
Gene Ontology Molecular Function
MRE11
Gene Ontology Biological Process
- DNA double-strand break processing involved in repair via synthesis-dependent strand annealing [IMP]
- DNA repair [IMP]
- ascospore formation [IMP]
- base-excision repair [IMP]
- double-strand break repair via break-induced replication [IGI, IMP]
- double-strand break repair via nonhomologous end joining [IMP]
- meiotic DNA double-strand break formation [TAS]
- meiotic DNA double-strand break processing [TAS]
- mitochondrial double-strand break repair via homologous recombination [IMP]
- reciprocal meiotic recombination [IMP]
- regulation of transcription during meiosis [IMP]
Gene Ontology Molecular Function- 3'-5' exonuclease activity [IDA]
- G-quadruplex DNA binding [IDA]
- adenylate kinase activity [IDA]
- double-stranded telomeric DNA binding [IDA]
- endodeoxyribonuclease activity [IDA]
- endonuclease activity [IDA]
- protein complex scaffold [IGI, IMP]
- single-stranded telomeric DNA binding [IDA]
- telomeric DNA binding [IDA]
- 3'-5' exonuclease activity [IDA]
- G-quadruplex DNA binding [IDA]
- adenylate kinase activity [IDA]
- double-stranded telomeric DNA binding [IDA]
- endodeoxyribonuclease activity [IDA]
- endonuclease activity [IDA]
- protein complex scaffold [IGI, IMP]
- single-stranded telomeric DNA binding [IDA]
- telomeric DNA binding [IDA]
Gene Ontology Cellular Component
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Genetic separation of Sae2 nuclease activity from Mre11 nuclease functions in budding yeast.
Sae2 promotes the repair of DNA double-strand breaks in Saccharomyces cerevisiae The role of Sae2 is linked to the Mre11/Rad50/Xrs2 (MRX) complex, which is important for the processing of DNA ends into single-stranded substrates for homologous recombination. Sae2 has intrinsic endonuclease activity, but the role of this activity has not been assessed independently from its functions in promoting Mre11 nuclease ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- double mutants show sensitivity to CPT (CHEBI:27656) and MMS (CHEBI:25255)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SAE2 MRE11 | Dosage Growth Defect Dosage Growth Defect A genetic interaction is inferred when over expression or increased dosage of one gene causes a growth defect in a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 1257303 | |
| MRE11 SAE2 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
| SAE2 MRE11 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 346185 | |
| SAE2 MRE11 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 163990 | |
| SAE2 MRE11 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 353039 | |
| SAE2 MRE11 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 2614447 | |
| SAE2 MRE11 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 1257302 | |
| SAE2 MRE11 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 3199782 | |
| SAE2 MRE11 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 3715106 | |
| MRE11 SAE2 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 3715107 | |
| MRE11 SAE2 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 2346202 | |
| SAE2 MRE11 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SAE2 MRE11 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| MRE11 SAE2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 824425 | |
| MRE11 SAE2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| MRE11 SAE2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 589492 | |
| MRE11 SAE2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 2199648 | |
| SAE2 MRE11 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 2533706 | |
| MRE11 SAE2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 2751636 | |
| SAE2 MRE11 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 2598345 | |
| SAE2 MRE11 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 1257299 | |
| SAE2 MRE11 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 2550073 | |
| SAE2 MRE11 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 3573380 | |
| SAE2 MRE11 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 1535226 | |
| MRE11 SAE2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID