BAIT

LCK

IMD22, LSK, YT16, p56lck, pp58lck, RP4-675E8.4
LCK proto-oncogene, Src family tyrosine kinase
GO Process (30)
GO Function (14)
GO Component (7)
Homo sapiens
PREY

PIK3R1

AGM7, GRB1, IMD36, p85, p85-ALPHA
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
GO Process (32)
GO Function (12)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of two SH3-binding motifs in the regulatory subunit of phosphatidylinositol 3-kinase.

Kapeller R, Prasad KV, Janssen O, Hou W, Schaffhausen BS, Rudd CE, Cantley LC

Src homology 3 (SH3) domains have been recently shown to bind to proline-rich sequences contained in 3BP1, 3BP2, and SOS. In a recent study we demonstrated that phosphatidylinositol 3-kinase (PI 3-kinase) associates with the Fyn SH3 domain. Here we show that p85, the regulatory subunit of PI 3-kinase, binds directly to the SH3 domains of Abl, Lck, Fyn, and p85 ... [more]

J. Biol. Chem. Jan. 21, 1994; 269(3);1927-33 [Pubmed: 8294442]

Throughput

  • Low Throughput

Additional Notes

  • p85, the regulatory subunit of PI 3-kinase, binds directly to the SH3 domains of Abl, Lck, Fyn, and p85 itself

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LCK PIK3R1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High30BioGRID
2994231

Curated By

  • BioGRID