BAIT

PRKCA

AAG6, PKC-alpha, PKCA, PRKACA
protein kinase C, alpha
GO Process (40)
GO Function (5)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens

Biochemical Activity (Phosphorylation)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Expression, purification and characterization of human PHD1 in Escherichia coli.

Li XY, Takasaki C, Satoh Y, Kimura S, Yasumoto K, Sogawa K

The hypoxia-inducible factors (HIFs) play a central role in oxygen homeostasis. HIF prolyl hydroxylases (PHDs) modify HIFalpha subunits and thereby target them for proteasomal degradation. Mammalian PHDs comprise three isozymes, PHD1, PHD2 and PHD3, and belong to the iron(II)-2-oxoglutarate-dependent dioxygenase family. We have expressed full-length human PHD1 in Escherichia coli, and purified it to apparent homogeneity by immobilized Ni-affinity chromatography, ... [more]

J. Biochem. Nov. 01, 2008; 144(5);555-61 [Pubmed: 18710826]

Throughput

  • Low Throughput

Curated By

  • BioGRID