BAIT

PHO84

phosphate transporter PHO84, phoT, L000001430, YML123C
High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
Saccharomyces cerevisiae (S288c)
PREY

PHO89

ITN1, YBR296C
Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Pho91 Is a vacuolar phosphate transporter that regulates phosphate and polyphosphate metabolism in Saccharomyces cerevisiae.

Huerlimann HC, Stadler-Waibel M, Werner TP, Freimoser FM

Inorganic polyphosphate (poly P) is a biopolymer that occurs in all organisms and cells and in many cellular compartments. It is involved in numerous biological phenomena and functions in cellular processes in all organisms. However, even the most fundamental aspects of poly P metabolism are largely unknown. In yeast, large amounts of poly P accumulate in the vacuole during growth. ... [more]

Mol. Biol. Cell Nov. 01, 2007; 18(11);4438-45 [Pubmed: 17804816]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chemical compound accumulation (APO:0000095)

Additional Notes

  • Deletion of PHO84 greatly reduces the cellular content of inorganic polyphosphate. Deletion of PHO87 and 89-91 has a small effect. Deletion of PHO84 and 3 or 4 of the others synergistically decreases inorganic polyphosphate levels.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHO84 PHO89
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
422876
PHO84 PHO89
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
482651
PHO89 PHO84
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
1028691
PHO84 PHO89
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
452531
PHO84 PHO89
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
161498

Curated By

  • BioGRID