BAIT

PHO84

phosphate transporter PHO84, phoT, L000001430, YML123C
High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
Saccharomyces cerevisiae (S288c)
PREY

PHO89

ITN1, YBR296C
Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae.

Ghillebert R, Swinnen E, De Snijder P, Smets B, Winderickx J

When starved for phosphate (Pi), yeast cells activate the PHO-signaling pathway, wherein the Pho4 transcription factor mediates expression of genes involved in Pi-acquisition, such as PHO84, encoding the high-affinity H+/Pi-symporter. In contrast, transcription of PHO87 and PHO90, encoding the low-affinity H+/Pi-transport system, is independent of phosphate status. Here, we reveal that upon Pi-starvation, these low-affinity phosphate transporters are endocytosed and ... [more]

Unknown Dec. 10, 2010; 0(0); [Pubmed: 21143198]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: utilization of phosphorus source (APO:0000125)

Additional Notes

  • genetic complex
  • triple mutants show a growth defect at in low phosphate media

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHO84 PHO89
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
258306
PHO84 PHO89
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
422876
PHO89 PHO84
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
1028691
PHO84 PHO89
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
452531
PHO84 PHO89
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
161498

Curated By

  • BioGRID